spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Klf5 constitutive expression prevents ESC differentiation. (A) ESCs that were mock-transfected (right panel) or transfected with Klf5 expression vector (left panel) were plated at low density (80 cells/cm2) and grown for 6 days without LIF. Scale bars: 1 mm. (B,C) Mock- or Klf5-transfected ESCs were grown for 5 days in differentiation conditions and stained with anti-Nanog and -Oct3/4 (B) or -Klf5 and -Nanog (C) antibodies. Scale bars: 100 µm. (D) Western blot analyses of Oct3/4 and Nanog in undifferentiated and differentiated ESCs. Protein extracts are from four clones stably expressing Klf5-overexpressed (Klf5 ov.) and one from mock-transfected cells.





Right arrow Return to article