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Figure 7


Fig. 7. Detection of tyrosine-phosphorylated Asef at lamellipodia and membrane ruffles. (A,B) A431 cells expressing HA-Asef (A) or HA-AsefY94F (B) were serum starved for 24 hours and then stimulated with 25 ng/ml EGF for 2 minutes. Cells fixed and stained using antibody against phospho-Tyr94 Asef (anti-pY94Asef) or anti-HA antibody were analyzed using a confocal microscope. (C,D) Fluorescence intensities of HA-Asef-WT (C) or HA-Asef-Y94F (D) are plotted along the red lines in A and B.





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