JCS023721 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A) qPCR analysis of cathepsin X mRNA in stably transfected and wild-type Jurkat T lymphocytes. mRNA levels obtained for wild-type Jurkat T lymphocytes have been normalized to relative concentration 1.0. Stable transfection of Jurkat T lymphocytes with pcDNA3/cathepsin X (clone 17) resulted in up to 71-fold increase of the cathepsin X mRNA expression level. (B) Real-time amplification plot. (C) Dissociation curves for cathepsin X qPCR reaction.

• Movie 1 -

  Movie 1. Migration and homotypic aggregation of wild-type Jurkat T lymphocytes on ICAM1-coated surface

• Movie 2 -

  Movie 2. Migration and homotypic aggregation of cathepsin X up-regulated Jurkat T lymphocytes on ICAM1-coated surface

• Movie 3 -

  Movie 3. Migration of wild type Jurkat T lymphocytes on Matrigel.

• Movie 4 -

  Movie 4. Migration of cathepsin-X-upregulated Jurkat T lymphocytes on Matrigel.