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Figure 2


Fig. 2. Homotypic cell aggregation of cathepsin-X-overexpressing T lymphocytes. Jurkat T lymphocytes were incubated for 24 hours in complete RPMI. (A) Wild-type Jurkat T lymphocytes show weak aggregation (B) Upregulation of cathepsin X in Jurkat T lymphocytes resulted in formation of large cell aggregates. Addition of EDTA, 2F12 cathepsin-X-neutralising mAb or cathepsin-X-specific inhibitor AMS36 reduced homotypic aggregation of cathepsin-X-overexpressing Jurkat T lymphocytes. Scale bars, 100 µm.





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