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Fig. 3. Somatic requirement of UNC-60A for organized assembly of actin networks in the myoepithelial sheath of the somatic gonad. rrf-1(pk1417) (RNAi-defective in soma) worms were treated with control RNAi (A-C) or unc-60A(RNAi) (D-F), and the dissected gonads were stained for F-actin (A,D) and DNA (B,E). (C,F) Merged images are shown (F-actin in red and DNA in blue). unc-60A(RNAi) resulted in the formation of abnormal clumps of oocytes but did not affect actin-filament organization in the myoepithelial sheath. Note that the micrographs in A-C are composites of multiple micrographs of the same gonad. Scale bar: 20 µm.