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Fig. 5. Different actin-filament-severing activities of the UNC-60A and UNC-60B variants. Fluorescently labeled actin filaments were tethered to a glass coverslip and treated with 10 nM to 10 µM of UNC-60A (A,B), UNC-60B(WT) (C,D), UNC-60B(
150) (E,F) or UNC-60B(
152) (G,H). The filaments were observed before (A,C,E,G) or 2 minutes after (B,D,F,H) the incubation, and the same fields are shown for each treatment. Scale bar: 10 µm.