JCS028977 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 (Adobe PDF) -

  Table S1. Strains used in this study.

• Supplemental Table S2 (Adobe PDF) -

  Table S2. Primers for the generation of gene replacement cassettes.

• Supplemental Table S3 (Adobe PDF) -

  Table S3. Primers for PCR identification of deletion mutants.

• Supplemental Figure S1 -

  Fig. S1. Morphology of wild-type cells at log phase in rich medium. The nuclear and general morphology was indistinguishable from that of DKO (Fig. 1C). Scale bar: 5 µm.

• Supplemental Figure S2 -

  Fig. S2. Conditioned minimal medium preferentially induces cell death in DKO cells that is not inhibited by mitochondrial inhibitor cyanide. (A) Time course of viability in the conditioned minimal medium. (B) Effect of cyanide on the viability of DKO cells in the conditioned minimal medium. Values shown are means ± s.e.m. from independent experiments (n>3).