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Figure 2


Fig. 2. Fatty acids, DAG and ceramide induce distinct forms of lipotoxic cell death in fission yeast. (A-C) Lipid profiles of wild-type and DKO cells at logarithmic phase (cell density of 1x107 cells/ml) and early stationary phase (6x107 cells/ml) in rich medium. A.U., arbitrary unit (defined in Materials and Methods). *P<0.05; **P<0.001. (D) Cell viability following oleic-acid treatment. (E) Cell viability following DAG and sphingolipid treatments. *P<0.05. DHS, dihydrosphingosine; PHS, phytosphingosine. All values shown are means with s.e.m. from independent experiments (n>=3). (F) Nuclear morphology following various lipid treatments. Scale bar: 5 µm.





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