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Figure 7


Fig. 7. Pca1 undergoes proteolytic cleavage that is dependent on cysteine 270. (A) Western blot of extracts of cells overexpressing C-terminally GFP-tagged pca1+. From the left: lane 1, vector control; lane 2, pca1+-GFP; lanes 3-5, three independent clones of pca1+-GFP with cysteine 270 specifically mutated to alanine. The same extracts were immunoblotted with serum raised against the N-terminus of Pca1 (B, left panel). In both cases, smaller bands corresponding to proteolytic products disappeared upon the mutation of the active cysteine 270. Untagged Pca1 (B, right panel) corresponded to the estimated full-length protein (~47 kDa).





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