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Figure 3


Fig. 3. CASK expression in HaCaT keratinocytes upon cell-cycle re-entry. (A) Serum-starved HaCaT cells were re-stimulated to enter the cell cycle by adding serum for 0, 6, 12, 22 or 30 hours, and cell-cycle analysis was performed at each time point to verify the stage of the cell cycle (graphs on right). Cells were co-stained with a rabbit polyclonal anti-CASK antibody (green channel) and a mouse monoclonal anti-Ki67 antibody (red channel) at each time point. Mitotic cells were seen at 30 hours (arrowheads). (B) Cells were harvested at the indicated times after each serum stimulation and immunoblotting was performed using antibodies against CASK (112 kDa) and {alpha}-tubulin (50 kDa). (C) Expression of cyclin A (60 kDa) and cyclin B (62 kDa) at indicated times after re-stimulation. Scale bars, 50 µm.





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