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Figure 7


Fig. 7. Proliferation, growth factor responses, apoptosis and basal integrin expression in siRNA-transfected synchronised HaCaT cells at 60 hours post siRNA transfection. (A) Cell-cycle analysis of DNA content in cells pulse labelled with EdU. Percentages of cells in G1 and S phases, and at G2 or M phase are indicated. (B) Control-siRNA- and CASK-siRNA-transfected cells were serum starved and left untreated, or were treated with 10 ng/ml KGF or 10 ng/ml TGF{alpha} for 24 hours. Proliferation was substantially enhanced in CASK-siRNA-transfected cells treated with KGF or TGF{alpha} for 24 hours compared with control-siRNA-transfected cells treated with the same growth factors. (C) Percentage of apoptotic cells. Cells were harvested and subjected to staining for apoptosis using an apoptosis detection kit (BD Biosciences). Positive-control cells provided in the kit show a high percentage of apoptosis compared with negligible levels seen in either the control or CASK siRNA transfected cells. (D) Basal integrin expression. Cells were harvested, stained for {alpha}6, β1 or β4 integrin and subjected to analysis by flow cytometry.





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