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Figure 9


Fig. 9. CASK expression during skin-wound healing. (A) H&E histology of day 3 mouse back-skin full-thickness wound (top panel). Boxed areas that were investigated for CASK expression are shown in magnification (bottom panel). Wound site (left); wound edge (middle); away from wound (right). Frozen sections of wounded skin were immunostained using a rabbit polyclonal anti-CASK antibody. Notice the reduced staining for CASK in keratinocytes at the wound site (WS). (B,C) Knockdown of CASK results in accelerated cell adhesion and spreading. HaCaT cells were transfected with control-siRNA or CASK-siRNA oligonucleotides for 96 hours and seeded onto collagen-coated substrates. After 30 minutes, 1 or 2 hours, attached cells were quantified by an adhesion assay (B) or stained for the focal adhesion marker vinculin (green) (C). DAPI nuclear staining is shown in blue. Scale bars, 50 µm (A), 20 µm (C).





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