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Fig. 8. ${Delta}$ N-TCF4 abolishes the effect of HGF in axon growth and branching. (A) Hippocampal neurons transfected with EYFP and ${Delta}$ N-TCF4, untreated or treated with BDNF, NT-3 and HGF (50 ng/ml). ${Delta}$ N-TCF4 abolishes the axon growth induced by HGF, but not by BDNF or NT-3. Scale bar: 40 µm. (B) Quantification of axon length in neurons expressing EYFP or EYFP plus ${Delta}$ N-TCF4 normalized to the respective untreated control shows that HGF and Wnt3a (used as positive control; 50 ng/ml) do not increase axon length in neurons expressing ${Delta}$ N-TCF4, whereas NTs similarly stimulate axon growth in ${Delta}$ N-TCF4-expressing or control neurons. (C) Quantification of axon branching in neurons expressing EYFP alone or EYFP and ${Delta}$ N-TCF4 shows that HGF-induced branching is inhibited by ${Delta}$ N-TCF4 expression, whereas the BDNF- and NT-3-induced effects are not affected (n=5-7 experiments). ^*P $≤$ 0.05, ^**P $≤$ 0.01, ^***P $≤$ 0.001, when compared with the corresponding untreated controls. #, P $≤$ 0.05; ##, P $≤$ 0.01; ###, P $≤$ 0.001, when compared with the corresponding stimulated control.

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