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Figure 5


Fig. 5. Exchange of PML protein variants at NBs. (A) Schematic depiction of PML-fusion proteins used for FRAP experiments. The domain structure is as described in Fig. 1. SUMO-modifiable lysine residues at positions 65, 160 and 490 in PML are also shown. (B-F) U-2 OS cells transfected with the indicated GFP-tagged PML protein constructs were used in FRAP analyses of areas that contain NBs and fluorescence recovery was monitored for various times as indicated. Scale bars, 5 µm. In C and F, cells were co-transfected with PML IV tagged to mRFP in order to analyze the impact of wild-type PML on the exchange dynamics of PML-IV–SUMO-2K (C) and PML-RAR{alpha} (F). (G-I) Graphs show mean values (± s.d.) from at least 20 FRAP experiments each, of the indicated proteins or protein combinations as relative fluorescence intensity (RFI) after normalization to pre-bleach levels.





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