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Fig. 7. Exchange dynamics of HIPK2, DAXX and BLM at PML NBs. FRAP experiments were performed in U-2 OS cells that express (A) GFP-tagged wild-type HIPK2, (B) GFP-HIPK2 in combination with mRFP–PML-IV (red), (C) the kinase-defective GFP-HIPK2(K221A) mutant in combination with mRFP–PML-IV (red), GFP-DAXX (D), and GFP-BLM (E). Areas containing HIPK2 accumulations were bleached and fluorescence recovery was recorded for various times as indicated. Scale bars, 5 µm. (F-I) Quantification of FRAP experiments as shown in A-E. Graphs show mean values (± s.d.) from at least 20 FRAP experiments each.