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Figure 2


Fig. 2. P311 increases lipid accumulation. (A,B) C3H10 control and cells stably expressing P311 were pulsed with fatty-acid complexes. Lipids were then extracted and measured for relative levels of triglycerides (A) and cholesterol (B). There is a significant (approximately threefold; P<0.05) increase in cholesterol and triglyceride levels in cells stably expressing P311. Mean ± s.d. from triplicate samples are shown. (C,D) Staining with the lipophilic dye Oil-Red-O of C3H10 control (C) and P311 stable (D) cells pulsed with fatty-acid complexes for 24 hours demonstrates the presence of lipid droplets in cells stably expressing P311 (D). (E-L) Phase-contrast (E,I,G,K) and epifluorescence (F,J,H,L) images of C3H10 cells stably expressing P311 (E-H) and NIH3T3-L1 adipocytes (I-L) immunostained with either adipophilin (E,F,I,J) or TIP47 (G,K,H,L) antibody demonstrating staining of lipid droplets in NIH3T3-L1 adipocytes with both adipophilin and TIP47 antibody, but no staining of lipid droplets in C3H10 P311 with either antibody. Scale bars, 5 µm (C,D).





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