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Figure 3


Fig. 3. The membrane proximal part of the C-terminus and the DRY motif of CCR7 are crucial for cell migration. Chemotaxis of 300-19 cells stably transfected with wild-type CCR7, MT1-3 or DNY mutants was assessed in TranswellTM chemotaxis assays. After 3 hours of incubation at 37°C, cells migrated in response to graded concentrations of CCL19 (A) or CCL21 (B) to the lower chamber were collected and counted by flow cytometry. Mean values ± s.d. of three independent experiments are depicted as percentage of input cells.





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