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Figure 2


Fig. 2. Disruption of the Golgi complex by overexpression of dominant-negative or wild-type Rab proteins. Cells on coverslips were transfected with a panel of wild-type or dominant-negative GFP constructs. Coverslips were fixed 24 hours post transfection. GM130 staining was visualized by immunofluorescence using a Cy3-tagged secondary antibody. Nuclei were visualized using DAPI. (A) Dispersal of GM130 staining in Vero cells (top) and COS7 cells (bottom) expressing wild-type GFP-Rab18. (B) Dispersal of GM130 staining in multiple COS7 cells expressing dominant-negative Rab43. Scale bars: 5 µm. (C,D) Quantification of Golgi dispersal in Vero cells expressing the indicated wild-type Arl/Rab GFP constructs (C) or corresponding dominant-negative constructs (D). (E,F) Quantification of Golgi dispersal in COS7 cells expressing the indicated wild-type Arl/Rab GFP constructs (E) or corresponding dominant-negative constructs (F). Golgi dispersal was quantified and normalized to Golgi dispersal in untransfected cells on the same coverslip. Error bars indicate s.e.m.





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