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Figure 3


Fig. 3. Light microscopic localization of Rab proteins to Golgi subdomains. NRK cells were transfected with the indicated GFP constructs. 24 hours post transfection, cells were fixed and stained for p115 and TGN38. The cis-Golgi marker p115 was visualized using a Cy5-tagged secondary antibody (blue) and the trans-Golgi marker TGN38 was visualized using a Cy3-tagged secondary antibody (red). GFP constructs were scored for localization within the Golgi as described (Dejgaard et al., 2007). (A) Schematic illustrating choice of location for linescans. (B) Examples of linescans analyzed. (C) Mean location values determined for the indicated constructs. Mean values for 20 cells/sample are shown. Error bars indicate s.e.m.





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