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Figure 4


Fig. 4. Dynamics of Golgi association of GFP constructs. NRK cells in glass-bottomed dishes were transfected with the indicated GFP constructs and time-lapse sequences taken 24 hours post transfection. (A) Frames from a typical time-lapse sequence from a NRK cell expressing GFP-Rab43 showing a prebleach image with typical Golgi and cytoplasmic labeling, ablation of Golgi fluorescence by photobleach and points in the recovery time sequence. (B) Recovery curve from cell shown in A. Golgi fluorescence as proportion of total cell fluorescence is plotted. (C) Mean half-times (seconds) for the indicated proteins. Error bars indicate s.e.m.





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