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Figure 5


Fig. 5. The Golgi complex is functional but relocated to ER exit sites in COS7 cells expressing Rab43-T32N. (A) Colocalization of GM130 (Cy3; red) with COPII (Cy5; blue) in cell expressing GFP-Rab43-T32N (24 hours post transfection). (B) VSVG-CFP transport to the cell surface in cells expressing GFP-Rab43-T32N (top, middle) or GFP-Rab43 (bottom). Cells were stained without permeabilization to visualize surface VSVG-CFP (Cy3; red) or with permeabilization to visualize total VSVG-CFP as indicated. (C) Total Golgi fragments in cells expressing the indicated GFP-Rab construct. Cells were transfected with the indicated GFP-Rab constructs and were fixed and stained 24 hours post transfection. Transfected cells were divided into three expression levels based on GFP fluorescence and Golgi fragments were counted. Data are mean ± s.e.m. (D) Dispersal of GFP-p150Glued from microtubules in cells expressing CFP-Rab43 (bottom). (E) Scoring of GFP-p150Glued localization to microtubules in singly transfected cells or cells transfected with CFP-Rab43, CFP-Rab43-T32N, Rab18 or Clontech CFP N1 vector. 100 cells were scored for each condition.





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