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Fig. 10. Expressing ZYX_LIM-GFP suppresses stretch-induced zyxin accumulation and actin polymerization at FAs. Cells transfected with either ZYX_LIM-GFP or GFP were grown on FN-coated elastic substrata and were treated with 100 µM blebbistatin for 30 minutes. The substrata were then uniaxially stretched as in Fig. 7. (A-D) Cells transfected with ZYX_LIM-GFP (A) or GFP (C) were stained for zyxin (B,D) after stretching of the substrata. Arrows in A indicate accumulated ZYX_LIM-GFP, and arrows in B and D indicate accumulated zyxin. Asterisks indicate the cells expressing the exogenous molecules. (E-H) Alexa568-actin (F,H) was introduced into cells transfected with ZYX_LIM-GFP (E) or GFP (G) after the stretching of substrata. Arrows in F and H indicate actin incorporated at peripheral FAs. Stretch-induced accumulation of ZYX_LIM-GFP at FAs could not be observed after introducing exogenous actin molecules, probably because the extent of the stretch-induced accumulation is low and the accumulated ZYX_LIM-GFP would be extracted from the cells during the introduction of actin molecules in the presence of digitonin. The double-headed arrow (bottom) indicates the direction of the stretch axis. Folds perpendicular to the stretch axis were generated as in Fig. 7. Scale bar: 20 µm.

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