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Fig. 2. Displacement of zyxin from FAs by expressing the isolated LIM region (338-572 aa) of human zyxin. (A-D) Cells were grown on FN and transfected with ZYX_LIM-GFP (A) or GFP (C). These cells were stained for endogenous zyxin (B and D, respectively). Endogenous zyxin was detected with an antibody against aa 134-150 of human zyxin. (E-J) Magnified images of cells numbered 1 (E,F), 2 (G,H) and 3 (I,J) in B. ZYX_LIM-GFP (E,G,I); endogenous zyxin (F,H,J). Scale bars: 20 µm. (K-M) Intensity profiles of the fluorescence images of ZYX_LIM-GFP (light-gray lines) and of endogenous zyxin (black lines) along the yellow lines in F,H and J are shown in K,L and M, respectively. (N,O) The averaged fluorescence intensity of endogenous zyxin at FAs was plotted against that of ZYX_LIM-GFP (N) or GFP (O) for each cell. Values were normalized with respect to the maximum value on each axis. CC, correlation coefficient. (P) Correlation coefficients of average fluorescence-intensity plots of the FA protein (zyxin, VASP, _v integrin or vinculin) vs ZYX_LIM-GFP (black bars) or GFP (gray bars). Fluorescence images and intensity plots for VASP, _v integrin and vinculin are shown in supplementary material Figs S3 and S4. Each bar represents the mean ± s.d. of three independent experiments. ^*P<0.05; ^**P<0.005 (t-test).

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