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Figure 4


Fig. 4. Talin and β1 integrin in a ligand-binding form accumulate at both peripheral and central FAs regardless of treatment with blebbistatin. (A-F) Cells grown on FN were treated with (D-F) or without (A-C) 100 µM blebbistatin for 30 minutes, and then fixed and double stained for {alpha}5 integrin (A,D, green in C,F) and talin (B,E, red in C,F). (G-J) Cells treated with (I,J) or without (G,H) 100 µM blebbistatin for 30 minutes were fixed and double stained with anti-{alpha}5-integrin antibody (G,I) and mAb B44, which recognizes the ligand-induced binding site (LIBS) on β1 integrin (H,J). Scale bars: 20 µm.





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