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Figure 7


Fig. 7. Interaction of N894D and N900D single-chain mutants with calnexin and EDEM. (A) Cell lysates from BHK-21 cells stably expressing CFTR, CFTR N894D, CFTR N900D or CFTR N894D/N900D were subjected to immunoprecipitation by incubation with anti-CFTR antibody 596 crosslinked to Dynabeads (IP: CFTR, as indicated on the left). CFTR and calnexin (CNX) were detected by western blotting using mouse monoclonal antibody 596 for CFTR or rabbit polyclonal antibody SPA860 for calnexin (as indicated on the right). Molecular weight marker positions (kDa) are indicated on the left. (B) CFTR was immunoprecipitated from cells that were stably expressing CFTR, CFTR N894D, CFTR N900D or CFTR N894D/N900D and that were also transiently expressing HA-tagged EDEM, by incubation with anti-CFTR monoclonal antibody 596 crosslinked to Dynabeads. EDEM was detected by western blotting using monoclonal antibody HA11 and CFTR using antibody 596.





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