JCS031195 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Effects of the length of the proximal region (L) and bend angle of imposed bending on the occurrence of the forward sliding (open circles) and the backward sliding (filled circles). The elastase-treated quiescent flagella were cut at the base (A) or not cut (B). After induction of the first sliding disintegration into two doublet bundles, imposed bending in the P-bend (left graphs) and R-bend (right graphs) directions induced backward sliding in A and B, respectively. The length of the proximal region (L) is defined as the distance between the proximal edge of the overlap of the two bundles and the point of imposed bending.

• Movie 1 -

  Movie 1. Original movie used for Fig. 3B showing sliding movement in a demembranated and elastase-treated quiescent flagellum. Photolysis of caged ATP by a UV flash (brighter image) induces splitting of the axoneme into two bundles of unequal thickness and the thinner bundle slides towards the head. After bending the distal part of the region of overlap in the P-bend direction with a microneedle further sliding occurs in the same direction as before (forward sliding).

• Movie 2 -

  Movie 2. Original movie used for Fig. 3C showing reversal of the sliding direction caused by bending in a demembranated, elastase-treated quiescent flagellum. UV-photolysis of caged ATP induces sliding of the thinner bundle towards the head. After bending the distal part of the region of overlap in the R-bend direction the direction of sliding reverses (backward sliding).