JCS031518 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. K14-Cre activation in utero. The K14-Cre transgene was crossed into the ROSA26 mouse line (Soriano, 1999). Cre activation in this line can be documented by staining for β-galactosidase (lacZ) activity. (A) lacZ activity in ROSA26/K14-Cre mice at embryonic day 14 (E14). The entire epidermis and the two developing hair follicles (arrows) are lacZ positive. (B) Wild-type control skin stained for lacZ activity.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Expression levels and subcellular distributions nuclear (Nuc)/cytoplasmic (Cyto) of selected signaling proteins in Dsc3^fl/fl/K14-Cre (Mut) and wild-type (Wt) control mice as determined by western blot analysis. The antibodies used are indicated. Expression of tubulin (cytoplasmic protein) and lamin B (nuclear protein) were used as loading controls. No significant differences were observed between mutant and wild-type samples.

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. Acantholysis (loss of cell−cell adhesion) between basal and suprabasal keratinocytes in the epidermis of newborn mice examined by electron microscopy. Desmosomes appear to be split in the middle (arrows). Half desmosomes with attached intermediate filaments are visible in the plasma membranes of cells on the bottom and roof of the blister cavity (star demarcates the blister cavity). Bar, 0.5 μm.