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Figure 1


Fig. 1. Schematic representation of the targeting strategy used to generate Dsc3fl (Dsc3tm2Ko) mice. (A) Exon 1 (ATG) of the Dsc3 gene was targeted with the vector shown in B. In the targeting construct, loxP sites were inserted in the promoter and intron 1. A neomycin minigene (PGK-Neo), flanked by FRT sites, was also inserted into intron 1. (C) The neo cassette was removed from recombinant ES cell clones via transient expression of FLPe recombinase, leaving a single FRT site in intron 1. (D) The resulting Dsc3fl/+ ES cells were used to generate mutant mice. (E) Cre-mediated recombination in bigenic mice (Dsc3fl/fl/K14-Cre) will lead to the deletion of exon 1, as well as part of the Dsc3 promoter and intron 1. (F) Newborn wild-type keratinocytes (Wt) synthesize both DSC3a and DSC3b, whereas Dsc3fl/fl/K14-Cre keratinocytes (Mut) do not express these proteins. The western blot shown was probed with a DSC3 antibody, stripped and re-probed with a β-actin antibody (loading control). Vertical bars indicate exons.





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