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Figure 2


Fig. 2. Characterization of newborn epidermis in mutant mice. (A) Newborn pup with severe ventral skin blistering. (B) Histology of an intra-epidermal blister in newborn mutant mice. Acantholysis is present immediately above the basal cell layer (star indicates blister cavity; bar, 100 µm). (C) Electron micrograph of a blister. Two half desmosomes are present in the plasma membranes of the acantholytic cells (double-headed arrow). The arrow indicates intermediate filaments that are attached to the half desmosome (bar, 0.2 µm). (D) Immunofluorescence microscopy using newborn skin of mutant mice (Mut) and wild-type (Wt) controls. The antibodies used are indicated. White stars indicate blister cavities. Junctional proteins are similarly distributed in both genotypes. ITGA6 ({alpha}6 integrin) or laminin staining (red) demarcates the basement membrane zone. The strong green fluorescence (DSC1, DSC3, DSG3 staining) is due to unspecific binding of the secondary antibody to the stratum corneum. Bars, 50 µm.





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