QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Defective vacuole and/or endocytic acidification causes a cell-wall defect. (A) vma1 ${Delta}$ , vma3 ${Delta}$ , btn1 ${Delta}$ and btn1 ${Delta}$ vma1 ${Delta}$ cells are sensitive to zymolyase. Enzyme sensitivity over time (minutes) of the indicated cells grown at 25°C. Cell lysis was monitored by measuring OD600 nm. (B) Acidic medium rescues zymolyase sensitivity and vacuole pH of btn1 ${Delta}$ cells. (i) Zymolyase sensitivity of wild-type cells or btn1 ${Delta}$ cells grown at 25°C in medium at pH 6 or pH 4. (ii) Vacuole pH as monitored by CDCFDA fluorescence. btn1 ${Delta}$ cells grown at 25°C in a medium of pH 6 are marked by post-staining with DAPI, those grown at pH 4 are unmarked. (C) Growth in acidic medium acidifies the vacuoles but differentially affects the zymolyase sensitivity of btn1 ${Delta}$ vma1 ${Delta}$ cells and those deleted for vma1 or vma3. (i) Zymolyase sensitivity after 90 minutes and (ii) zymolyase sensitivity over time of indicated cells grown in medium of pH 4 or pH 6 at 25°C. Unpaired Student's t-tests were performed (n=3, ^*P<0.05, ^**P<0.01, ^***P<0.001). (D) Vacuole pH as monitored by CDCFDA fluorescence of the indicated cells grown in a medium of pH 4 or pH 6 at 25°C. Typical results are presented for A, Bi and Cii. Scale bars: 10 µm.

Return to article