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Fig. 1. (A-T) TRPV4 expression in kidney and inner ear. In MDCK cells, TRPV4 immunofluorescence was preferentially localized at the basolateral membrane (arrows in A). TRPV4 was also localized at the basolateral membranes (arrows) of wild-type (+/+) murine kidney distal tubule cells, but not in adjacent glomerular regions (g, in B), nor in the kidney of TRPV4-deficient mice (–/–, in C). In wholemounts of the stria vascularis from wild-type mice, TRPV4 was localized at the lumenal surface of marginal cells (m in D; green in E) at the level of the actiniferous tight junctions (red) between adjacent marginal cells (E, same focal plane as D). There was negligible TRPV4 immunoexpression at the lumenal surface of marginal cells of TRPV4-deficient mice (F). In a focal series of the cells shown in D and E, below the actiniferous tight junctions, only weak TRPV4 immunofluorescence occurred in the somata of marginal cells, but was prominent at the periphery of marginal cells (i in G, green in H). There was no TRPV4 expression detected in the somata of marginal cells from TRPV4-deficient mice (I). At lower focal planes than in G and H, strong, characteristic TRPV4 expression (J,M, green in K,N) was localized in intermediate (i) cells. TRPV4 expression could not be detected in the intra-strial tissues of TRPV4-deficient mice (L,O). In z-sections of the stria vascularis, TRPV4 immunolabeling was clearly associated with the lumenal surface (arrows) of the marginal cells (m), between the actiniferous tight junctions, and in the intermediate cells (i) below the weakly-labeled somata of marginal cells of wild-type mice (P,Q). In the organ of Corti, TRPV4 expression was most prominent in the region of tight junctions between adjacent pillar cells (arrowheads), and also between adjacent Deiters' cell phalangeal apices (arrows; R,S). The stereocilia of inner hair cells displayed TRPV4 expression (double arrowheads; R,S). TRPV4 expression was also present in Deiters' cell phalanges (d), but only weakly in OHCs (ohc) and spiral ganglion cells (T). Scale bar in C also applies for A and B. Scale bar in O also applies for D-N, R-T. Scale bar in Q also applies for P.
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