spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. GTTR uptake is enhanced in the absence of extracellular Ca2+. (A,B) In the presence of extracellular 1.25 mM Ca2+, KPT2 and KDT3 cells had modest and low levels of intracellular GTTR fluorescence, respectively, after 30 seconds of GTTR treatment. (C,D) In the absence of extracellular Ca2+, KPT2 cells showed a modest increase in GTTR fluorescence (C), whereas KDT3 cells showed substantially enhanced GTTR uptake (D). In both KPT2 and KDT3 cells, GTTR fluorescence was diffusely distributed in the cytoplasm, with small puncta (arrowheads) within the nucleus. (E) Fluorescence intensity of cytoplasmic GTTR in KDT3 cells was significantly lower than in KPT2 cells in the presence of extracellular Ca2+ (**P<0.01). Removal of extracellular Ca2+ greatly enhanced GTTR fluorescence in both KPT2 and KDT3 cells compared with 1.25 mM Ca2+ condition (P<0.001). The enhanced GTTR levels by extracellular Ca2+ removal were similar between KPT2 and KDT3 cells. Fluorescence intensity data are shown as mean ± s.d. Scale bar, 20 µm.





Right arrow Return to article