JCS031641 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Reduction of RalA or RalB expression does not substantially alter steady-state levels of assembled Exocyst complex in prostate tumor cells. R3327-5′A cells or R3327-5′A cells expressing shRNAs targeting RalA or RalB were extracted in 1% Triton X-100. Extracts were subjected to immunoprecipitation with antibodies to Sec8. Presence Sec8, Sec5, Sec15 and Exo84 in equivalent amounts of whole cell extracts (total) and precipitated immune complexes (α-Sec8 IP) was assessed by SDS-PAGE. Indicated proteins were identified by immunoblotting with specific antibodies, and protein levels were quantified using a Molecular Dynamics Typhoon phosphorimager. Band intensities for each protein pair were calculated and normalized to levels present parental 5′A cells, which is defined as ‘1’.