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Fig. 2. P-Rex1 distribution during hippocampal differentiation. (A) One day in vitro (d.i.v.) embryonic rat hippocampal neurons were immunostained with P-Rex1-specific antibodies (green), Rac antibodies (red), Texas Red-conjugated phalloidin (red) or β-tubulin antibodies (red) as indicated. Higher-magnification images of the boxed area outlining the growth cones are shown in the fourth column. Arrows indicate endogenous P-Rex1, Rac or F-actin at tips of growth cones. Co-localisation in merged images appears yellow. (B,C) Three (B) and 4-7 (C) d.i.v. embryonic rat hippocampal neurons were co-stained with P-Rex1-specific antibodies (green), Texas Red-conjugated phalloidin (red), β-tubulin (red), Tau1 (red) or MAP2 (red) antibodies. The relative intensity of P-Rex1 antibody staining is shown in the left-hand column by `glowover' images, in which blue indicates high-intensity staining (see scale beneath B). Merged images in the right-hand column demonstrate co-localisation by yellow staining. Open arrows indicate primary neurites. Arrowhead indicates P-Rex1 co-localisation with β-tubulin. White arrows in C indicate Tau1-positive or MAP2-negative processes. Scale bars: 10 µm in A; 20 µm in B,C.

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