JCS032417 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Normal outgrowth of Dpr2-deficient blastocysts. The wild-type (WT) (A-D) and Dpr2^−/− (E-H) blastocysts were derived from Dpr2^+/− intercrosses and explanted at embryonic day 3.5 (E3.5) (day 0). After culture for 4 days, both types of blastocysts hatched from the zona pellucida and developed outgrowths normally. The outgrowths were cultured for 16 hours in the presence of 50 μM BrdU. BrdU incorporation was visualized by BrdU immunofluorescence (D,H). The nuclei were stained blue with DAPI (C,G). Scale bar: 200 μm.

• Supplemental Figure S2 -

  Fig. S2. TGF-β1 expression in day 0, 3 and 5 wounds of wild-type (WT) and Dpr2^−/− mice was analyzed by western blotting with anti-TGF-β1 antibody. β-actin was used as a loading control.

• Supplemental Figure S3 -

  Fig. S3. Analyses of hair follicle morphology and hair structure of Dpr2-knockout mice. (A,B) Sections of Hematoxylin and eosin-stained skin of wild-type (WT) (A) or Dpr2-knockout mice (B). Note that hair follicles in Dpr2^−/− mice looked normal. (C-F) Inner structures of zigzag (C,D) and awl (E,F) hairs of wild-type (C,E) and Dpr2^−/− mice (D,F) were observed under a light microscope. Scale bar: 100 μm (A,B), 50 μm (C-F).