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Figure 5


Fig. 5. Enhanced TGFβ signaling in Dpr2–/– keratinocytes. (A) Reporter gene assay. Dpr2–/– and wild-type (WT) keratinocytes were transfected with (CAGA)12-luciferase or cotransfected with constitutively active forms of ALK5 (caALK5) or mouse Dpr2 (mDpr2). Next day, the cells were treated with or without TGFβ1 (Tβ1, 5 ng/ml) for another 20 hours. Results are relative luciferase activity of transfected cells compared with the Renilla control. All values are expressed as mean ± s.d., n=3, *P<0.05, **P<0.01. (B,C) Western blot analyses of protein extracts from Dpr2–/– and wild-type keratinocytes for ALK5 (B) and phosphorylated Smad2 (Smad2-P) (C). Cells treated with or without TGFβ1 (Tβ1) for 48 hours. β-actin or total Smad2 served as loading controls. Bar charts show densitometry results. All values are mean ± s.d., n=3; **P<0.01.





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