spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Expression levels of integrins are upregulated in Dpr2–/– keratinocytes. (A-D) Dpr2–/– and wild-type (WT) keratinocytes were treated with TGFβ1 for the indicated time. Real-time RT-PCR was carried out using RNA isolated from each type of cells. Integrin (Itg) β6 (A), β1 (B), {alpha}v (C) and {alpha}5 (D) gene expression was quantified and normalized to HPRT, and relative values expressed as mean ± s.d. of at least four independent experiments. (E) Adhesion of Dpr2–/– keratinocytes was stronger than that of wild-type keratinocytes. Wild-type and Dpr2–/– keratinocytes were treated with TGFβ1 (5 ng/ml) for 48 hours, and the harvested cells were regrown in wells coated with fibronectin (50 µg/ml). The optical density at 550 nm of retained cells stained with Crystal Violet is shown. Data are means ± s.d. of three independent experiments; *P<0.05, **P<0.01.





Right arrow Return to article