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Figure 3


Fig. 3. CAP1 is directed to mitochondria through the N-terminal domain. (A) GFP fusion constructs. (B) Translocation of GFP fusion CAP1 functional domains to mitochondria after apoptosis induction. HEK293T cells were transfected with the indicated CAP1constructs. 24 hours after transfection, cells were treated with 1 µM STS for 2 hours. Gradient-purified mitochondria were subjected to western blotting with anti-GFP. VDAC blotting was used to normalize total proteins from mitochondrial fractions.





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