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Figure 3


Fig. 3. Immunolocalization of endogenous TIP47, ADPH[fl]-VSV and {Delta} 2,3 ADPH-VSV. (A) Immunostaining of endogenous TIP47 (green) and ADPH[fl]-VSV (red) or {Delta} 2,3 ADPH-VSV (red) following a 24-hour incubation of 293 cells (a,d,g,j), {Delta} 2,3 ADPH-VSV cells (b,e,h,k) and ADPH[fl]-VSV cells (c,f,i,l) in basal media (Cont, a-c), 300 µM OA (d-f), 5 µM MG132 (MG, g-i) or 300 µM OA plus 5 µM MG132 (OA + MG, j-l). Panels a,d,g,j were immunostained with chicken anti-human TIP47; b,e,h,k and c,f,i,l were immunostained with mouse anti-VSV and chicken anti-human TIP47. (B) Enlargement of regions in Ad-f are shown (a-c) to better illustrate CLD staining properties of endogenous TIP47, {Delta} 2,3 ADPH-VSV and ADPH[fl]-VSV. Nuclei were stained with DAPI (blue). All experiments were performed at least three times, with similar results. Multiple images were captured in each experiment; representative images are shown. Images were originally taken at 600x magnification. Scale bar: 20 µm.





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