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Figure 4


Fig. 4. Specificity of N-terminal control of ADPH stability. (A,B) Schematics of ADPH-TIP47-VSV and TIP47-ADPH-VSV chimeras. (C,D) Representative immunoblots of ADPH-TIP47-VSV (C) and TIP47-ADPH-VSV (D) chimeras following a 20-hour incubation of their respective cultures in basal culture media (control) or in basal culture media supplemented with 3 µM MG132 (MG), 300 µM OA, or 3 µM MG132 plus 300 µM OA (MG/OA). Chimeras were detected by probing with mouse anti-VSV antibodies; blots were stripped and reprobed for β-actin to control for loading. (E,F) Quantitation of the respective immunoblots in C and D. The values are averages ± s.d. for three samples, each normalized to β-actin. The experiment was repeated twice, with similar results. Statistical analyses of these results are given in the text.





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