|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Activated β-catenin expression during in vivo skeletal muscle regeneration. (A-P) Acute muscle regeneration following cardiotoxin injection of the tibialis anterior muscle in mouse. (A-H) 6 days after injection. (I-P) 15 days after injection. (A) Haematoxylin and eosin staining showing heterogeneously sized muscle fibres containing centrally located nuclei. (B) Enlarged region of adjacent section to that in A showing Act-β-Cat localisation to centrally (red arrowhead) or peripherally (yellow arrowheads) located nuclei. (C) Act-β-Cat localisation to centrally (red arrowhead) or peripherally (yellow arrowheads) located nuclei within laminin-expressing myofibre. (D) Clusters of robustly expressing Pax7-positive cells (green arrowheads). (E) Weak expression of Caveolin 3 to mark the regenerating sarcolemma. Note variable fibre diameter and shapes. (F) High-power confocal image showing expression of Act-β-Cat (yellow arrowheads), (G) the nucleus (red arrowheads) and (H) an overlay showing predominantly nuclear localisation of Act-β-Cat (white arrowheads). (I) 15 days after injury, haematoxylin and eosin staining showed muscle with mostly uniform sized fibres with very few displaying centrally located nuclei. (J) Weak expression of Act-β-Cat located outside of the nuclei (yellow arrowhead). (K) Localisation of Act-β-Cat adjacent to laminin expression. (L) Pax7 expression becomes infrequent. (M) Robust expression of Caveolin 3. On rare occasions, Act-β-Cat was found adjacent to a nuclei but high-power confocal imaging showed its expression (N) in relation to the nucleus (O) was complimentary (P). (Q-F1) Human muscle regeneration. (Q-T) Chronic muscle regeneration. (Q) Variably sized fibres with centrally located nuclei. (R) Nuclear localisation of Act-β-Cat (white arrowheads). (S) Centrally (red arrowheads) and peripherally (yellow arrowhead) located nuclei showed expression of Act-β-Cat. (T) Robust expression of Pax7 (green arrowheads). (U-X) Control human muscle showing no signs of regeneration. (U) Haematoxylin and eosin staining showing peripheral nuclei and a lack of tissue infiltrate. (V) Sparse non-nuclear Act-β-Cat expression (green arrowheads). (W) Rare Act-β-Cat within laminin-positive muscle fibres (green arrowhead). (X) Rare Pax7 expression beneath muscle fibre lamina (green arrowhead). (Y-B1) Chronic human muscle regeneration. (Y) Robust Pax7 and Ki67 double-positive nuclei showing dividing satellite cells (yellow arrowheads). (Z) Overlay of image shown in Y with DAPI confirming nuclear localisation (white arrowheads). (A1) Strong expression of Act-β-Cat in Ki67-positive cells (yellow arrowheads). (B1) Overlay of the image shown in A1 with DAPI confirming nuclear localisation of the Act-β-Cat and Ki67 signal (white arrowheads). (C1-F1) Control human muscle. (C1) Rare expression of Pax7 in a quiescent satellite cell negative for Ki67 (green arrowhead). (D1) overlay of the image shown in C1 with DAPI confirming the nuclear localisation of Pax7 (white arrowhead). (E1) Rare pockets/membranous regions of Act-β-Cat expression are noted along with Ki67-negative muscle tissue (green arrowhead). (F1) Overlay of the image shown in E1 with DAPI, confirming the non-nuclear localisation of Act-β-Cat (green arrowhead).
|
