JCS025684 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Postsynaptic infusion of CaN-AIP converts synaptic facilitation toward depression with inter-spike interval at 100 ms. (A) Responses of a unitary synapse from presynaptic pyramidal neuron and postsynaptic fast-spiking neuron vs time under postsynaptic infusion of CaN-AIP (40 µM). uEPSC-1 (red symbols) rises, and uEPSC-2 (blue symbol) decreases. (B) Changes in the probability of uEPSC-1 (red symbols) and uEPSC-2 (blue symbols) over 0-3 minutes and 35-38 minutes of the postsynaptic infusion of CaN-AIP. CaN-AIP raises the probability of uEPSCs. (C) Changes in the amplitude of uEPSC-1 and uEPSC-2 over 0-3 minutes (white bars) and 35-38 minutes (gray bars) of postsynaptic infusion of CaN-AIP. CaN-AIP raises the amplitudes of uEPSCs. (D) Patterns of this unitary synapse (uEPSC2-uEPSC1) vs time while infusing CaN-AIP (40 µM) postsynaptically. The patterns of this unitary synapse shift toward depression. (E) Waveform of uEPSCs (top traces) and spikes (bottom traces) over 0-3 minutes of postsynaptic infusion of CaN-AIP. (F) Waveform of uEPSCs (top traces) and spikes (bottom traces) at 35-38 minutes of postsynaptic infusion of CaN-AIP.