JCS022368 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Adenophostin-A-induced synaptic potentiation occludes tetanus-induced LTP. (A) Compared with the control (open symbols), the infusion of adenophostin A (100 nM) induces a robust potentiation of synaptic transmission, and subsequent addition of tetanus (100 Hz, ten pulses repeated ten times per minute) does not induce further potentiation, except for short-term potentiation. (B) The averaged data (n=9) show that adenophostin-A-induced synaptic potentiation occludes tetanus-induced LTP.

• Supplemental Figure S2 -

  Fig. S2. Bath application of mGluR1 agonist (DHPG) elevates intracellular Ca²⁺ in cortical neurons. Ca²⁺ imaging was carried out after ACSF containing DHPG was perfused onto cortical slices every 20 seconds. Top-left panel shows the measurement of a Ca²⁺ transient in a pyramidal neuron in the control. Bottom-left panel shows the measurement of a Ca²⁺ transient in this pyramidal neuron during the bath application of 50 µM DHPG. Top-right panel presents the quantitative changes of Ca²⁺ transient in this pyramidal neuron under DHPG perfusion (red line) and in the control (green line). Bottom-right panel shows the net change in the Ca²⁺ transient upon application of DHPG (blue line).

• Supplemental Figure S3 -

  Fig. S3. Tetanus and mGluR1 agonist (DHPG) elevate intracellular Ca²⁺ in cortical neurons. (A) Ca²⁺ imaging of control neurons. (B) Ca²⁺ imaging of neurons during high-frequency simulation (100 Hz, ten pulses repeated ten times per minute). (C) Ca²⁺ imaging in a control pyramidal neuron. (D) Ca²⁺ imaging in the pyramidal neuron during adenophostin A infusion (100 nM). (E) Quantitative changes of Ca²⁺ transient in cell 2 before (green line) and after (red line) treatment with tetanus. (F) Quantitative changes of Ca²⁺ transient in cell 4 before (green line) and after (red line) treatment with tetanus. (G) Quantitative changes of the Ca²⁺ transient in a control (green line) and adenophostin-A-infused (red line) pyramidal neuron.