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Figure 3


Fig. 3. Distribution of syntaxins and SNAP23 in PC12-27 cells, relative to the enlargeosome marker desmoyokin. The four series of deconvolved confocal images, obtained from PC12-27 cells permeabilized before immunolabeling, illustrate: (left) the distribution of Stx4, Stx6 and Stx13 (A-C, respectively) and of SNAP23 (D); (middle) that of desmoyokin; (right) the merge of the two corresponding images. All the syntaxins and SNAP23 decorate punctae that are spread in the cytoplasm, with different patterns. In all cases the co-labelling of these SNAREs with desmoyokin is very low and below significance (<10%), except for SNAP23 in the area near the plasma membrane. In addition, Stx6 and also Stx13 show intense labelling in the perinuclear area. Notice the intense signalling of Stx13 and SNAP23 at the periphery of the cells. Scale bar: 5 µm.





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