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Figure 3


Fig. 3. Changes in mRNA steady-state levels following heat shock. Cell cultures were transferred from 27°C to either 37°C or 41°C over a time course, and RNA was analysed by northern blotting and microarrays. All probes used in this work were specific (supplementary material Fig. S2A). (A) Northern blot probed for total mRNA using an antisense oligonucleotide recognising the spliced leader; the spliced leader RNA (SLRNA) is indicated. One of three replica gels is shown, all three were used for quantification; error bars indicate ± s.d. (B) Oligonucleotide microarray slides were probed with RNA from cells incubated at either 27°C or 41°C. The total dataset is shown as a plot of the intensities for each spot. (C) The filtered dataset from B. (D) Northern blots probed for PDI-2, actin and {alpha}- and β-tubulin. Data from one representative experiment out of several repeats are shown. (E) Northern blots probed for HSP70 (Tb11.01.3110) and HSP83 (Tb10.26.1080). One out of two replica gels is shown. Quantification from both replica gels is shown.





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