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Figure 6


Fig. 6. Characterisation of T. brucei heat-shock stress granules. (A) Homologues of stress granule markers were expressed as eYFP fusion proteins. Fluorescent images of unstressed (27°C) and heat-shocked (1 hour 41°C) cells are shown. (B) The effect of heat shock on cells that express PABP1-eYFP from a modified endogenous locus; treatments are indicated. (C) Disappearance of heat-shock stress granules after a return to 27°C in cells that express PABP1-eYFP from a modified endogenous locus. The percentage of cells with visible granules was determined during recovery and the average values (n=100) from three slides is shown, error bars indicate ± s.d. (D) Optical sections through T. brucei cells that express PAPB1-eYFP or eIF4E3-eYFP after 60 minutes at 41°C are shown as projection (left) and some selected single plane images (right). (E) Colocalisation of eIF3B-eYFP and eIF4E3-mChFP to heat-shock stress granules.





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