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Figure 8


Fig. 8. Association of VAPA and VAPB with polymerised microtubules. (A) Detergent-solubilised extracts of transfected cells were pulled down with polymerised microtubules. The `input' panels show 25% of the input signal. A-WT, wild-type VAPA; A-{Delta}N, N-terminal deletion VAPA; A-P56S, mutant P56S VAPA; B-WT, wild-type VAPB; B-{Delta}N, N-terminal deletion VAPB. (B) Quantitative analysis of VAPA and VAPB recovered with polymerised microtubules in the presence of AAAT or FFAT peptide at the indicated concentrations. Values were normalised to the amount of VAPA or VAPB recovered in the absence of either peptide. Filled symbols represent microtubule association of VAPA-WT, VAPA-P56S and VAPB-WT in the presence of AAAT peptide whereas open symbols represent association of VAPA-WT, VAPA-P56S and VAPB-WT in the presence of FFAT peptide.





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