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Figure 5


Fig. 5. Zoids and 1K2N cells appear to be produced as siblings. Cells induced for TbCentrin4-RNAi for 24 hours were fixed and labelled with DAPI (nucleus, kinetoplast), 20H5 (basal bodies and bi-lobe) and anti-GRASP (Golgi). (A) A cell near the end of cytokinesis, separating into one daughter cell that contains two nuclei, and the other daughter cell without a nucleus. (B) A cell just after division, one daughter cell with two nuclei, the other with none. (C,D) Both siblings contain basal bodies (open arrowheads), a kinetoplast (arrows), a bi-lobe (closed arrowheads; the large arrowhead indicating the lobe that is associated with the single Golgi stack, the small arrowhead indicating the other lobe) and a Golgi complex (red). Scale bar, 5 µm.





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