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Figure 3


Fig. 3. Abl and its PxxP motif interaction partners modulate Rac1 activity during attachment. NIH3T3 cells overexpressing CrkII, Nck2, Abl or 1234 (A), STI-571 treated or NIH3T3 cells with knockdown of Crk or Nck (B) were harvested in suspension or 5 minutes after attachment on 4 µg/ml fibronectin-coated dishes. Whole cell lysates (WCL) were subjected to GTPase assay, and the amounts of Rac1 and Cdc42 were detected by western blotting.





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