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Figure 7


Fig. 7. C4ST1 overexpression renders astrocytes far less permissive to neuronal growth. Wild-type or inactive form of C4ST1 was transfected into astrocytes and neurons were co-cultured on monolayers. Expression levels of recombinant C4ST1 (A) and 4-sulfated GAG chains (B) were examined in immunoblot with anti-DsRed antibody and LY111 (anti-4-sulfated GAG), respectively. Internal cleavage of DsRed fusion protein was detected. (C) Disaccharide composition analyses of conditioned medium (CM) were performed as in Fig. 5. (D) Relative axonal length was measured. (*P<0.01 compared with vector; ANOVA.) Axonal growth was reduced by increased production of 4-sulfated GAG chains in astrocytes.





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